The Protective Roles of Mito-TEMPO on Testicular Ischemia‑Reperfusion Injury Based on Biochemical and Histopathological Evidences in Mice

Accumulation of reactive oxygen species during testicular torsion causes oxidative stress, which in turn causes ischemia-reperfusion (I/R) injury to the testis. In testicular torsion/detorsion (T/D) in male mice, the purpose of this study was to investigate the influence of Mito-TEMPO (MT) on I/R injury. Forty-two male mice were divided into seven groups, including a control group and six treatment groups (۳۶۰° T/D, ۷۲۰° T/D, ۳۶۰° T/D + ۰.۷۰ mg/kg MT, ۳۶۰° T/D + ۱ mg/kg MT, ۷۲۰° T/D + ۰.۷۰ mg/kg MT and ۷۲۰° T/D + ۱ mg/kg MT). After inducing testicular torsion, oxidative enzymes, and testicular histopathology were evaluated. The results showed that ۷۲۰° T/D resulted in increased testicular malondialdehyde levels and histological damage, along with reduced activities of catalase, superoxide dismutase, and glutathione peroxidase. Treatment with MT reduced tissue malondialdehyde levels and increased the activities of catalase, superoxide dismutase, and glutathione peroxidase. These findings suggest that MT administration protects against acute testicular T/D injury in mice.

Keywords:

Mito-TEMPO , Ischemia‒reperfusion injury , oxidative stress , testicular histopathology