Efficacy of Salvia Officinalis Extract Against Infertility in Oxidative Stress Conditions

This study aimed to investigate the effects of Salvia officinalis L. extract on infertility of male rats. In this experimental study, ۴۰ rats were used in ۸ groups and cadmium chloride was also used to induce infertility. S. officinalis extract was fed to mice daily for ۲۸ days with the help of gavage needle. Animals were anesthetized with ether and dissected, then epididymal sperm were examined for morphology, viability and motility. we analyzed their right testis for H/E staining and counting cell lines and their left testicles were analyzed to evaluate superoxide dismutase (SOD) and malondialdehyde (MDA) enzymes.According to the results of three sperm quality analyses indicated a significant increasing of viability, motility and the number of sperm cells in infertile groups treated with ۱۰۰, ۲۰۰, and ۴۰۰ mg/kg S. officinalis extract (P<۰.۰۰۱). Our SOD analysis revealed Salvia Officinalis extract caused to produce more enzymes in the healthy experimental groups, even compared to the healthy control group (P<۰.۰۰۰۱). our analysis indicated that increasing the dosage of the S. officinalis extract resulted in increasing enzyme production in infertile groups. MDA analyses showed that the healthy experimental groups produced a lower amount of MDA enzyme, which is almost the same level as the healthy control group (P<۰.۰۰۰۱). Due to the usage of S. officinalis extract, the level of lipid peroxidation and MDA significantly decreased. In this study, S. officinalis extract can have significant positive impacts on the viability, motility, number of sperms in oxidative stress conditions.

Keywords:

Salvia officinalis , Infertility , Cadmium chloride , oxidative stress