Lentil (lens culinaris Medik.) genotype × PGR (Plant growth regulator) interaction and heritability of some traits under in vitro conditions

Plant breeders are constantly evaluating genetic diversity resources to lay a foundation for crop improvement. Tissue culture techniques have been applied successfully in many species to generate genetic variation in plants. Researches on lentil genetic diversity and parameter estimation under tissue culture conditions are relatively limited. Therefore, this research was carried out in three separate experiments for genetic parameters estimation and to understand the response of ۱۴ lentil genotypes to callus induction using kinetin (Kin) and ۲,۴-dichlorophenoxyacetic acid (۲,۴-D) growth regulators; shoot production using thidiazuron (TDZ), ۲-Isopentyladenine (۲iP), benzylamino purine (BAP), and Kin growth regulators; and rooting the plants using NAA growth regulator. Results showed that the best treatments were ۱ mg/L Kin and ۲ mg/L ۲,۴-D for callus induction, ۳ mg/L BAP, ۴ mg/L Kin, and ۲ mg/L TDZ for shoot regeneration, and ۳ mg/L NAA for rooting. The ۰۹S ۸۳۲۵۹-۱۴ILL۶۹۹۴/ILL۵۴۸۰ and FLIP۲۰۱۰-۴۰L-۱۰۷۷۰-ILL۸۱۱۹/ILL۷۶۸۶ genotypes were the best in term of callus, shoot, and root induction. The broad sense heritability of most of the traits was high demonstrating lower contribution of environmental factors in phenotypic variances. Overall, large diversity identified among genotypes in vitro conditions and high heritability estimates indicated that selection based on the measured traits including callus, shoot, and root induction will be efficient in tissue culture conditions.

Keywords:

Genetic parameters , Heritability , Plant growth regulators , Regeneration