Preparation and evaluation of the effects of drug delivery nanosystem against Staphylococcus aureus by microbroth dilution and well diffusion methods in laboratory conditions

Introduction: A hospital infection is an infection that occurs after the patient is admitted to the hospital (۴۸ or ۷۲ hours later) or during a certain period (۱۰ to ۳۰ days) after the patient's discharge (۲۵ to ۵۰% of surgical wound infections. Drug delivery systems can transfer their medicine to the target site in different forms. Staphylococcus aureus is the most dangerous positivespherical bacteria (cocci) often cause -common staphylococcal bacteria. These gramskin infections, but can also cause pneumonia, heart valve infections, and bone infections. Gentamicin is an aminoglycoside antibiotic used to treat several types of bacterial infections. According to the given explanations, the aim of the study is to Preparation and evaluation of the effects of drug delivery nanosystem against Staphylococcus aureu by microbroth dilution and well diffusion methods in laboratory conditions Method: Firstly, mesoporous silica nanoparticles were prepared. Loading mesoporous silica nanoparticles with drugs were done and nanoparticle coating using chitosan. The antibacterial activity of the studied groups against Staphylococcus aureus (ATCC:۲۳۲۳۵) was performed by diffusion method and according to CLSI protocol. Results: The Morphological and surface characteistics of the synthesized nanoparticles were investigated with a scanning electron microscope. Loading efficiency and loading capacity of gentamicin-MSN nanoparticles are ۹۹.۹۹±۰.۰۱ and ۱۹.۹۶±۰.۰۱. The size of the ۱۰۰ nm-particles was about ۵۰. Conclusion: Combination therapy and efficient drug delivery systems have been developed to treat infections. Formula showed more antibacterial activity compared to free forms and vancomycin. These carriers can improve antimicrobial activity and also reduce the required effective concentration and induce rapid bacterial clearance.

Keywords:

Gentamicin , Nanomedicine , Drug delivery , Staphylococcus aureus