The effect of hydro-alcoholic extract of Eryngium caeruleum on pentylenetetrazol (PTZ)- induced seizures in male mice

Introduction: Seizure is one of the most important symptoms of epilepsy and many other neurogenic injuries. Due to the chronicity of epilepsy and the side effects of chemical drugs and drug resistance, with the aim of achieving effective treatment methods and less adverse effects, use of medicinal plants has attracted a lot of attention. The aim of this study was to investigate the effect of hydro-alcoholic extract of Eryngium caeruleum on pentylenetetrazol (PTZ)-induced seizures in male mice. Methods: In this experimental study the NMRI male mice were randomly divided into six groups of ۸ each. Forty-five minutes before the injection of PTZ (۸۰ mg/kg) as a convulsive agent, hydro-alcoholic extract of Eryngium caeruleum (۱۰۰, ۳۰۰, ۵۰۰ and ۱۰۰۰ mg/kg; to the treatment group), saline (۱۰ ml/kg; to the negative control group,) or Phenobarbital (۴۰ mg/kg; to the positive control group) were injected. All injections were done intraperitoneally (IP). The initiation time of myo-clonic and tonic-clonic seizures and death percent within ۲۴ h were measured and P<۰.۰۵ was considered significant. Results: The results showed that different doses of the extract delayed the onset of myo-clonic and tonic-clonic convulsions and reduced the percentage of ۲۴-hour mortality compared to the control group, which were significant in the doses of ۳۰۰, ۵۰۰ and ۱۰۰۰ mg/kg. Conclusion: It seems that the hydro-alcoholic extract of Eryngium caeruleum presented decremental effect on PTZ-induced seizure and death in male mice.

Keywords:

Eryngium caeruleum , Pentylenetetrazol , seizure , mice