Whole sequence analysis of BRCA۱ Gene to Unravel the Genetic Diversity and Phylogenetic Structure of the Domestic and Wild Sheep

The BRCA۱ gene, which is called “gate keeper” It plays an important role in the process of repairing DNA damage, maintaining chromosome stability, regulating the cell cycle and regulating the transcription process. The current research was conducted with the aim of bioinformatic analysis and comparison the genetic diversity and phylogenetic structure of the BRCA۱ gene of different breeds of domestic and wild sheep from ۴۵ breeds in ۶ geographical regions using the NCBI genome database. The desired sequences were aligned using MEGA۱۱ software and a phylogenetic tree was drawn by Neighbor-Joining method. Also, nucleotide diversity, haploid diversity, the number of mutations, the percentage of gene differentiation and gene conversion were analyzed using Dnaspv۵ software. The analyses related to the comparison of breeds showed ۷۳۵ polymorphisms, which led to the creation of ۷۵ different haplotypes with a haplotype diversity of ۰.۰۳۵. Nucleotide diversity and average nucleotide differences (k) among breeds were estimated as ۰.۲۰۵.

Keywords:

BRCA۱ gene , genetic diversity , phylogenetic structure , domestic sheep , wild sheep , bioinformatic analysis