The Impact Of Aerobic Exercise Combined With Lactobacillus Supplementation On The Expression Of Adiponectin And Appl۱ Genes In The Liver Tissue Of Wistar Rats With Fatty Liver Disease

Objective: The present study aimed to investigate the effects of aerobic exercise combined with Lactobacillus supplementation on the expression of adiponectin and APPL۱ genes in the liver tissue of rats induced with fatty liver. Materials and Methods: A total of ۳۲ rats with an average weight of ۲۲۰±۲۰ grams were divided into ۴ groups: ۱. FLCG (Fatty liver control group), ۲. FLLSG (Fatty liver with Lactobacillus supplementation group), ۳. FLAEG (Fatty liver with aerobic exercise group), ۴. FLAELS (Fatty liver with combined aerobic exercise and Lactobacillus supplementation group). The first group was fed a standard rodent diet, while the fatty liver groups were converted into a model group by receiving tetracycline via gavage. In the exercise and exercise ± supplementation groups, aerobic training was conducted on a treadmill for ۶ weeks, ۵ days a week. The supplementation groups received ۱۰^۹ CFU/ml of Lactobacillus rhamnosus GG daily via gavage for ۵ weeks. Surgery and liver biopsy were performed at the end of the study. The expression levels of adiponectin and APPL۱ genes in liver tissue were measured using Real-Time PCR technique. All data from this study were analyzed using SPSS version ۲۴. Two-way ANOVA and Tukey's post-hoc test were used for intergroup comparisons, with a significance level set at p<۰.۰۵. Results: The findings regarding adiponectin gene expression indicated no significant difference between the control group and the supplementation group (p=۰.۴۰۱). However, both aerobic exercise and aerobic exercise with supplementation groups showed significant differences compared to the control group (p=۰.۰۰۱). Thus, aerobic exercise combined with Lactobacillus supplementation significantly affects adiponectin gene expression in the liver tissue of rats with induced fatty liver. Similarly, results for APPL۱ gene expression showed no significant difference between the control and supplementation groups (p=۰.۲۰۰), but both aerobic exercise and aerobic exercise-supplementation groups exhibited significant differences compared to the control group (p=۰.۰۰۱). Therefore, aerobic exercise combined with Lactobacillus supplementation significantly impacts APPL۱ gene expression in the liver tissue of rats with induced fatty liver. Conclusion: The results of this study indicate the effective role of aerobic exercise and Lactobacillus consumption on the expression of adiponectin and APPL۱ genes in liver tissue in laboratory samples of fatty liver disease. If the exercise regimen is sufficiently intense and prolonged, it can play a major role in treating fatty liver.

Keywords:

Aerobic exercise , Lactobacillus , Adiponectin , APPL۱ , Fatty liver