Fluorescence resonance energy transfer between protein and CdS quantum dots coated by polyacrylamide grafted onto salep

The polyacrylamide grafted onto salep-functionalized CdS quantum dots (CdS-PAAm-g-salepQDs) were synthesized in aqueous solution using safe and low-cost precursors. The TEM results of CdS-PAAm-g-salepQDs showed that the average size of QDs was about 3.5 nm. Fluorescence resonance energy transfer (FRET) system wasconstructed between CdS-PAAm-g-salep QDs (acceptor) and protein (human serum albumin (HSA) and bovine serumalbumin (BSA)) (donor) in aqueous solution. The energy transfer efficiency (E) and the distance (r) between HSA (orBSA) and CdS-PAAm-g-salep QDs were obtained.

Keywords:

FRET , Polyacrylamide , Distance , HSA , BSA