Simultaneous production of animal feed enzymes (endoxylanase and endoglucanase) by Penicilliumjanthinellum from waste jute caddies

Background: In West Bengal, India, a huge amount of lignocellulosic jute waste products, in the form of unutilizedcut pieces of jute caddies consisting 65% cellulose, 22.5% hemicellulose, and 11% lignin, is generated from juteindustries. Naturally, the high percentage of lignocellulolytic material made it an attractive substrate for thehydrolytic cleavage of lignocellulolytic enzymes, namely xylanases and cellulases. The present study aims for theproduction of xylanolytic and cellulolytic enzymes by a fungal isolate Penicillium janthinellum MTCC10889 using acheap medium containing residual jute caddies obtained from jute mill wastes and chemically defined basalmedium under submerged fermentation.Results: The fungal isolate P. janthinellum MTCC10889 is an outstanding producer of endoxylanase and alsocellulase. A central composite design was applied to optimize its lignocellulolytic as well as cellulolytic enzymeproduction in submerged-state fermentation. The impact of three quantitative variables, namely pH, temperature,and substrate concentrations, on enzyme production was investigated by using a chemically defined basal mediumsupplemented with jute caddy as substrate. Such optimum conditions were as follows: pH 7.47, temperature of30.35°C, and substrate concentration of 1.70% for endoxylanase, and pH 7.51, temperature of 29.69°C, and substrateconcentration of 2.11% for cellulase production.Conclusions: A combination of one-factor-at-a-time approach is replaced by response surface methodology usingcentral composite design, which resulted in 3.08- and 3.84-fold increases in the yields of endoxylanase (1,750 IU/ml)and endoglucanase (192 IU/ml), respectively. The highest endoxylanase and endoglucanase productions underoptimized conditions were achieved within 48 h of growth.

Keywords:

Central composite design , Endoxylanase , Endoglucanase , RSM , Penicillium janthinellum