Spectrometric Determination of Amitriptyline from RealSamples Using a Two Liquid-phase Microextraction Method

The main aim of this research is using of the preconcentration and sample preparation method, based on two liquid phase microextraction procedure of amitriptyline prior to UV-Vis spectrophotometer. Here, the target compound was extracted from an aqueous sample solution (donor phase) into an organic solvent drop (acceptor phase). Several important parameters which influence the extraction efficiency such as thekind of extracting solvent, volume of the organic acceptor phase, extraction time, stirring rate, pH of the donor phase and salt effect were investigated. Under the optimal conditions the enrichment factor was 54. By plotting absorbance versus concentration of the analyte in the standard solutions, calibration curve was obtained with correlation coefficient of 0.9984. The linearity of the method has been investigated between the ranges of 0.04-1 μg mL-1. The limit of detection (LOD) of the method which calculated theoretically was 0.013 μg mL-1. The precision or repeatability of the method is based on the average relative standard deviation (RSD %) is 3, for three different concentrations of the analyte. Finally, the proposed method was applied for the determination of amitriptyline in real water samples and the relative recoveries atspiked level of 0.1 μg mL-1 were upper than 91 %.

Keywords:

Two liquid-phase microextration (LLME) , Amitriptyline , UV-Vis spectrophotometer , Wateranalysis