Physiological groups of heterotrophic community bacteria present at River Actopan, Chachalacas barrier, Veracruz

The aim of this study was to determine physiological groups of heterotrophic community bacteria in River Actopan mouth, Chachalacas, Veracruz, Mexico. Water samples were collected in sterile jars in three zones of river: zone A (ÚrsuloGalván bridge), zone B (La Loma de San Rafael) and zone C (La Bocana). The water samples were analyzed through specific culture mediums for heterotrophic bacteria groups that degrade collagen, cellulose, chitin, starch and hydrocarbons. Species were identified by conventional biochemical tests, identification strips API 20E and 20NE and for the case of bacteria that degrade hydrocarbons it was used the sequencing of RNA gen 16S. The results indicate that in zone A, bacteria that use collagen and starch as source of carbon were most abundant (105.6 cfumL-1 and 100 cfumL-1 respectively), in zone B, collagen and chitin bacteria obtained 71.6 cfumL-1 and 60 cfumL-1, respectively. While in zone C, higher values were shown in gelatinases and cellulose degradative bacteria with 109 cfumL-1 and 106 cfumL-1 respectively, although it was also observed that this zone has more abundance in oil degrading bacteria with respect to zone A and B.

Keywords:

Heterotrophic , bacteria , Actopan River , Veracruz , Pollution , Coastal area