Determination of genetic diversity of type IV pili among Pseudomonas aeruginosa isolated from burn patients of Guilan province
Publish place: First National Conference on New Findings of Microbiology
Publish Year: 1394
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
FCNFM01_045
تاریخ نمایه سازی: 2 تیر 1397
Abstract:
Introduction: Type IV pili (TFP) are important colonization factors of the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa), involved in biofilm formation and attachment to host cells. P. aeruginosa TFP are divided into five distinct phylogenetic groups. Each pilin allele is stringently associated with distinct accessory genes that allow identification of the allele by specific PCR. The invariant association of the pilin and accessory genes implies horizontal transfer of the entire locus. This study undertook an analysis of TFP alleles in burn isolates of P. aeruginosa.Material and Methods: In this study 50 isolates were collected from burn patients in Velayat hospital, Rasht, Guilan province. 35 isolates were identified as P. aeruginosa using morphological and biochemical methods. The prevalence of pilA, tfpO, tfpY, tfpW and tfpZ genes in P. aeruginosa strains were amplified by PCR using specific primers.Results: The results showed that 26 out of 35 isolates had tfpY gene, 4 isolates tfpZ, 1 isolate tfpO gene, 4 isolates did not have any accessory genes that it means they have group II pilins of TFP and none of the isolates had tfpW gene.Conclusion: In conclusion, the prevalence of isolates with group III pilin genes that contained tfpY as accessory gene were more than other strains in burn patients. This information could be useful for comprehensive pilin-based vaccine design.
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Authors
Sareh Khanali
NourDanesh Institute of Higher Education, Meymeh, Isfahan,IR Iran
Iraj Nikookar
Laboratory of Microbiology and Immunology of Infectious Diseases, Paramedicine Faculty, Guilan University of Medical Sciences, Guilan, IR Iran
Bagher Yakhchali
NourDanesh Institute of Higher Education, Meymeh, Isfahan,IR Iran,Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IR Iran
Sobhan Faezi
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IR Iran