In Vitro Antifungal Activities of Grizeofulvin and silver nanoparticles on T.mentagrophytes

Introduction: Dermatophytosis is the most commen skin infection caused by dermatophytic fungi, such as Trichophyton spp. Invasive fungal disease represents a major threat to life in immunocompromised patients and is now one of the most common causes of infection in this group. There is a limited range of antifungal agents available to treat disease caused by trichophytones including the polyenes, Griseofulvin, azoles and more recently the echinocandins. Therefore it is necessary to find new ways of treatment for Trichophyton mentagrophytes. The purpose of this study was to investigate the antifungal effects of Grizeofulvin and silver nanoparticles on T.mentagrophytes in vitro.Material and Methods: Different concentrations of Griseofulvin and silver nanoparticles prepared. Antifungal effects of Griseofulvin and silver nanoparticles against T.mentagrophytes investigated by agar dilution method. Then minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) determined by the brothmicro dilution method. Results: The results of the agar dilution showed that silver nanoparticles at 160 ppm and drug at 125 μg/ml can inhibit the growth of T.mentagrophytes. In the broth microdilution method MIC and MFC for the silver nanoparticles 7.8 ppm and 15.62 ppm were obtained, and for Griseofulvin MIC was equal to 7.8 μg/ml and MFC was 15.62 μg/ml. Conclusion: Effects of Silver Nanoparticles according to their size was almost the same with Griseofulvin, But because of treatment failure with chemical drugs, numerous side effects and drug resistance led to the discovery of new therapeutic regimens. Based on this study and other studies in the field of silver nanoparticles due to strong germicidal effects and low toxicity to human cells in the body and high stability is a good candidate for treatment.

Keywords:

Silver nanoparticles , Griseofulvin , T.mentagrophytes , Antifungal activity.Antifungal Activities , Silver nanoparticles , Trichophyton mentagrophytes , Griseofulvin