Screening Effective Factors in Slurry Phase Bioremediation of TNT Contaminated Soils
Publish place: 06th International Congress on Chemical Engineering
Publish Year: 1388
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICHEC06_394
تاریخ نمایه سازی: 1 مهر 1388
Abstract:
Explosive contaminated soils are recognized as a major environmental issue due to the toxic and mutagenic effects of these energetic materials. Particularly, 2,4,6-trinitrotoluene (TNT), a common military explosive, has a main role in explosive contaminated soils. Bioremediation has recently emerged as an alternative solution to conventional physical and chemical methods for treatment of organic contaminant such as TNT. In this work, after selection of the superior bacterium among four bacteria in aqueous phase, slurry phase bioremediation of clay soil contaminated with 1000 mg/kg TNT was carried out in shaking flasks, showing the maximum potential of 97% TNT removal by this bacterium after 25 days. As a first step in TNT elimination optimization in slurry phase, the effective factors have been screened via the two-level fractional factorial design method plus three center points using seven factors i.e., glucose (0.2, 8%), yeast extract (0, 0.02%), (NH4)2SO4 (0.01, 0.05%), Tween80 (0.1, 0.5%) and slurry concentrations (20, 40%) as well as inoculum size (5, 10%) and temperature (20, 35˚C) during 15 days. It was found that slurry, surfactant and glucose concentrations and inoculum size are the significant factors. In addition, cross (mutual interaction) interactions of glucose with other effective factors were considerable.
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Authors
Gelareh Sheibani
Biotechnology Research Laboratory, School of Chemical Engineering, Iran University of Science and Technology, PO. Box ۱۶۸۴۶-۱۳۱۱۴, Tehran, Iran
Fereshteh Naeimpoor
Biotechnology Research Laboratory, School of Chemical Engineering, Iran University of Science and Technology, PO. Box ۱۶۸۴۶-۱۳۱۱۴, Tehran, Iran
Parisa Hejazi
Biotechnology Research Laboratory, School of Chemical Engineering, Iran University of Science and Technology, PO. Box ۱۶۸۴۶-۱۳۱۱۴, Tehran, Iran
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