Study on UV-visible for detection of biosynthesis of silver nanoparticles by oyster mushroom’s extracts

Twelve extracts belong to four species of edible oyster mushrooms were screened for their chemical value (viz. proteins, carbohydrates and phenols were assessed) and their capability to biosynthesize silver nanoparticles (AgNPs). In limitative conditions, dark incubation and temperature 25 °C, three modes of extracts preparation were developed. Properties of silver nanoparticles creation from extracts solution with 1 mM AgNO3 were investigated by color and UV-Visible spectroscopy to confirm silver nanoparticles formation. The bright yellow oyster mushroom (Pleurotus cornucopiae var. citrinopileatus) was finding as a potential candidate for the synthesis of AgNPs. Brown color of aqueous solution was given indication for AgNPs formation. Results showed that AgNPs absorption band was located at a peak of 440 nm for P. cornucopiae var. citrinopileatus. Although others P. ostreatus (grey & white) and P. salmoneostramineus (pink) were not form AgNPs due to no change in color of extracts with Ag ions when incubated under the same conditions, which indicative for no silver nanoparticles synthesis. Thus, AgNPs formation depended on species of oyster mushroom, method of extracting, concentration of extract and the conditions of incubation (of light and temp.), but no on content of proteins, carbohydrates and phenol in crude extract leastwise in this study.

Keywords:

Pleurotus spp. , Chemical value , Extraction method , Silver nanoparticles , Nanotechnology