A Simple Hydrogen Peroxide Biosensor Based on a Novel Electro Magnetic Polymer@Fe3O4 Nanocomposite

Fast and easy determination and measurement of hydrogen peroxide (H2O2) is of practicalimportance in clinical, pharmaceutical, biochemical, environmental [1,2]. In recent years,researchers have focused on magnetic nanoparticles. Due to their good biocompatibility, strongsuperparamagnetic properties, low toxicity and easy preparation processes, magneticnanoparticles have been used in various fields, including enzyme immobilization [3]. However,the problems of aggregation and rapid biodegradation limited applications of magneticnanoparticles to biosensing [4]. One of the most notable candidates for improvement of suchpractical applications may be nanomaterials based on conducting polymers. In the present study,Fe3O4 nanoparticles and conductive carboxy methyl cellulose/Fe3O4/CNT-COOH (CMC-Fe3O4-CNT-COOH), synthesized by organic chemistry group, were combined to develop animmobilization of horseradish peroxidase enzyme (HRP) for the determination of H2O2 by usingelectrochemical methods. The proposed biosensor showed good reproducibility and highsensitivity to H2O2 with the detection limit of 0.63 μM (S/N=3). In the range of 3.0–80.0 μM, thecatalytic reduction current of H2O2 was proportional to its concentration. The apparentMichaelis–Menten constant of HRP on the CMC-Fe3O4-CNTCOOH nanocomposite wasestimated to be 0.58 μM.

Keywords:

Hydrogen Peroxide , Horseradish Peroxidase , Nanoparticle