Fabrication of Rapid Diagnostic Strips for Detection of Influenza Virus
Publish place: 14th Conference on Biophysical Chemistry
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
CBC15_101
تاریخ نمایه سازی: 29 خرداد 1398
Abstract:
Influenza is unique among viral infections because of its propensity for seasonal epidemics and occasional pandemics, and because of the morbidity and mortality that result from its pulmonary complications. The need for a timely diagnosis, which allows for optimal use of these treatments, led to the introduction of numerous rapid diagnostic tests. Herein, we report on a lateral flow immunoassay (LFIA) for influenza A antigen using colloidal gold nanoparticles (AuNPs) as reporters. The matrix protein of influenza A virion (one of its most abundant structural proteins) was used as a model to demonstrate a performance of the LFIA. The spectroscopic properties of colloidal AuNPs showed the typical surface plasmon resonance band at 525 nm in UV-visible spectrum. Using dynamic light scattering (DLS) spectroscopy, the binding and homogeneity of golds were confirmed. The optimum pH, antibody concentration for conjugation and optimum concentration of antibodies on strips were 9, 60 μg/ml and 100 μg/ml, respectively. Under optimized conditions, the nanogold-based LFIA is capable of detecting virus as low as 256 pfu·mL-1 using a sample volume of 20 μL, within 20 min, and without interference by other proteins.
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Authors
Maede Abedini
Malek-Ashtar University of Technology, Tehran, Iran
Asieh Aramvash
Malek-Ashtar University of Technology, Tehran, Iran
Azadeh Azizi
Malek-Ashtar University of Technology, Tehran, Iran
Frida Behzadian
Malek-Ashtar University of Technology, Tehran, Iran