Synthesis of a Novel Dendrimer Based on Poly Ethylene Glycol for Gene Transfection into the HEK-293T Cells
Publish place: 26th Iranian Seminar on Organic Chemistry
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
View: 323
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ISOC26_112
تاریخ نمایه سازی: 2 شهریور 1398
Abstract:
The delivery of the gene is a process in which external DNA is introduced into the host cell.Different methods have been developed for gene transfer in a variety of cells and tissues, frombacteria to humans. In order to carry out a good gene transfer, the outer DNA needs to survivein host cells so that it can integrate with its gene [1]. Gene transplantation in therapeutic programsuses non-immune carriers that are appropriate to the cell s characteristics, which cantransmit enough transgene expression and result in an arbitrary effect [2]. In this paper, wesynthesized a novel dendrimeric carrier based on a novel functionalized polyethylene glycol(PEG) to access the immunity and minimum of toxicity. The addition of step-by-step of ethylenediamineand methyl methacrylate to the functionalized PEG. Then, the dendrimeric carrierwas used to transfer the plasmid to HEK-293 T cells.
Authors
Mina Islami
Department of chemistry, Graduate University of Advanced Technology, Kerman, Iran
Elaheh Mosaddegh
Department of New Materials, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, PO Box ۷۶۳۱۵-۱۱۷, Kerman, Iran
Masoud Torkzadeh-Mahani
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, PO Box ۷۶۳۱۵-۱۱۷, Kerman, Iran