Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 22، Issue: 9
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
View: 378
This Paper With 12 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-22-9_014
تاریخ نمایه سازی: 20 مهر 1398
Abstract:
Objective(s): To address the alarming problem of methicillin-resistant Staphylococcus aureus (MRSA), herein, a marine Streptomyces capable of producing an anti-MRSA compound has been studied.Materials and Methods: Strain MN41 was morphologically and physiologically characterized and then, molecularly identified using 16SrRNA analysis. To produce the bioactive compound in large scale, a kind of submerged liquid fermentation was adopted. The antibacterial agent was purified using a silica gel column followed by a semi-preparative HPLC and the isolated metabolite was identified using mass spectrometry, Nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR). Finally, the production process was subjected to a two steps optimization using Plackett-Burman design (PBD) and Response Surface Method (RSM), respectively. In addition, the antitumor activity of the active agent was studied.Results: The purified compound with a molecular weight of 421.2 was identified as a natural pyrrole-derivative. The optimization revealed a significant effect for starch, pH, calcium carbonate and peptone on the production of this anti-MRSA compound and resulted in a 218% increase in the production yield. Conclusion: The isolated pyrrole-derivative showed a remarkable activity against MRSA and also showed some promising anti-tumor activity.
Keywords:
Authors
Hamed Norouzi
Department of Biology, University of Isfahan, Isfahan, Iran
Mohamad Rabbani khorasgani
Department of Biology, University of Isfahan, Isfahan, Iran
Abolghasem Danesh
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :