Analysis of transcriptome profiling of responsive somatic cells to UV-B irradiation in the colonial green alga Volvox carteri
Publish place: The Third International and 15th National Genetics Congress
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
CIGS15_407
تاریخ نمایه سازی: 13 بهمن 1398
Abstract:
Light is one of the most important environmental signal that affects the physiology and action of Volvox carteri. However, there are limited reports about the effects of UV-B irradiation on genome-wide transcriptional regulation in this multicellular green alga. V. carteri has only 2 cell types includes mortal, motile somatic cells and immortal, immotile reproductive cells. In addition, it is a suitable organism for transcriptomics studies since its compact genome with a relatively small number of genes. Here, using RNA-seq data, we surveyed the overall transcriptional responses of two cell-types, somatic cells and reproductive cells, to continuous UV-B irradiation. Gene expression analysis was carried out in order to elucidate the effect of UV-B radiation on whole transcriptional modification of physiological mechanisms. The results showed that, as compared to control group, there were no differentially expressed genes in reproductive cells under treatment. However, treating the somatic cells with UV-B radiation resulted in at least 126 differential genes as compared to untreated control group. Our results showed that there is light-specific transcriptional regulation in this organism. So that, different pathways (i.e., metabolic process, cellular process, response to stimulus, localization, cellular component organization or biogenesis, biological regulation, regulation of biological process) were co-regulated by UV-B irradiation via the transcriptional regulation of genes encoding key enzymes in these pathways.
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Authors
s ekhtari
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
j razeghi
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
k hasanpur
Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
a Kianianmomeni
Department of Cellular and Developmental Biology of Plants, Faculty of Natural Sciences, University of Bielefeld, Bielefeld, Germany
a Movafeghi
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran