Comparative analysis of virulence genes, antibiotic resistance and capsule locus polymorphism of Enterococcus faecalis isolated from canals of root-filled teeth with periapical lesions with those from different clinical infections and fecal flora
Publish place: 20th International Congress of Microbiology of Iran
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM20_028
تاریخ نمایه سازی: 26 بهمن 1398
Abstract:
Introduction and Objectives: Enterococcus faecalis (E.faecalis ) is considered normal flora in human but has been considered a leading cause of hospital-acquired infections. In the oral cavity, it is commonly detected from canals of root-filled teeth with periapical lesions. The aim of this study was to compare antibiotic resistance patterns, the presence of virulence factors and capsule locus polymorphism among E. faecalis isolates recovered from different sources. Materials and Methods: Eighty-eight E. faecalis isolates recovered from canals of root-filled teeth with apical periodontitis (n=22), human blood and CSF as invasive isolates (n=22), urine and wound as non-invasive isolates (n=22) and fecal flora of healthy individuals (n=22) were examined. Resistance to different antibiotic agents was determined by disk diffusion method. Phenotypic method was used to determination of gelatinase production and biofilm formation. Polymerase chain reaction technique was used to detection of esp,ace, ebp, gelE, cyl, cps1, cps2,cps5 and cbh genes. Result: The antibiotic susceptibility of the isolated dental, invasive, non-invasive and fecal E.faecalis showed the presence of multidrug resistant isolates. This property was significantly more common in non-invasive compared with fecal isolates (P= 0.022). Antimicrobial resistance rates were not significantly different between isolates from root canals and fecal flora (p˃0.05). There were significantly different among isolates from root canals and fecal flora in gelatinase production (p= 0.009) and strong biofilm formation (p = 0.003). E. faecalis isolates from root canals carried cyl gene at a significantly higher frequency than invasive infections (p =0.002) and fecal flora (p =0.035). The presence of the esp gene was also significantly different between root canals isolates and the other isolates (p=0.000) .The most common type of capsule locus polymorphism among the root canals isolates was CPS 1 (63%) which suggests the lack of essential genes in cps operon for capsule production in these isolates . Conclusion: This study demonstrated that there are major genetic differences between the E. faecalis isolates from root canal infections and those from clinical and normal flora. Knowledge about the characterizations of E. faecalis isolated from different sources may help to find appreciated procedures preventing enterococcal infections.
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Authors
Roya Ahmadrajabi
Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Arash Shahravan
Endodontology Research Center, Kerman University of Medical Sciences, Kerman, Iran
Fereshteh Saffari
Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Mohammad Hossein Sobhanipoor
Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran