Molecular identification of Candida isolates obtained from HIV/AIDS patients by Real time PCR- High Resolution Melting Analysis

Introduction and Objectives: Candida species are the major important cause of opportunistic mycoses. Due to different antimycotic resistance patterns in various Candida spp, reliable and fast techniques for their identification and quantification are needed. High-resolution melting real-time PCR (PCR-HRM) is a new strategy for rapid diagnosis and characterization of Fungal Infections. In this study, we assayed Real-time PCR and HRM for the rapid identification of 46 Candida isolates obtained from oral cavity of HIV/AIDS patients. Materials and Methods: In this study, 46 Candida isolates obtained from oral cavity of HIV/AIDS patients were identified by Real-Time PCR and high-resolution melting curve analysis. Result: Considering reference and clinical isolates, 46 Candida species distinguished by Real-time PCR and HRM. Identified Eight species of Candida spp included 14 C. albicans (30.43%), 12 C. kefyr (26.09%), 11 C. glabrata (23.91%), 2 C. krusei (4.35%), 2 C. tropicalis (4.35%), 2 C. lusitaniae (4.35%), 2 C. guilliermondii (4.35%) and 1 C. parapsilosis (2.17%). Conclusion: HRM is a reliable and simple approach for identifying important Candida species. It should be considered as a diagnostic method.

Keywords:

Candida spp , High resolution melting , HIV/ADIS