Molecular Typing of Klebsiella pneumoniae isolates using Repetitive Extragenic Palindromic Sequence-Based PCR in Kurdistan province, Iran

Introduction and Objectives: Klebsiella pneumoniae is one of the main causes of hospital infection and a member of Enterobacteriaceae family. The main purpose of the present study was determine the repetitive extragenic palindromic polymerase chain reaction (rep-PCR) pattern among Klebsiella pneumoniae isolates in Kurdistan province, Iran. Materials and Methods: During a period from October 2015 to July 2016, 70 Klebsiella pneumoniae isolates were collected from different clinical specimens from general hospitals in Kurdistan province, Iran. The genetic relation of isolates was performed using rep-PCR typing assay. Results: 27clusters distinguished by the result of fingerprinting by rep-PCR. this pattern of isolates showed a wide diversity of clinical isolates in Kurdistan province, Iran. Conclusion: the present study revealed the emergence and spread of Klebsiella pneumoniae isolates with diverse genetic backgrounds in a hospital in Kurdistan province.

Keywords:

Klebsiella pneumoniae , repetitive extragenic palindromic polymerase chain reaction (rep-PCR) typing , Kurdistan province