Investigating the expression of PIP1, PIP2, and GAI genes in dwarf and vigorous genotypes of walnuts

Use of dwarf genotypes or rootstocks in fruit orchards increase garden performance. Several genes are involved as candidate genes for dwarfness. Two paths were investigated for dwarfness creation in walnut genotypes. The gibberellin signal transduction pathway and the ability to absorb and transfer of water by Acupoinin proteins. The ability of Aquaporins to increase the permeability of the membrane to water may affect the growth of cells, organs and plants. This hypothesis has been tested by measuring the gene expression changes in growth period. The expression of three genes in two dwarf and vigorous walnut genotypes was performed at three different times. The results of gene expression analysis showed that the expression of GAI, PIP2 and PIP1 genes in the three tissues studied at the first time of May did not have a significant difference between the dwarf genotypes. The results of the mean comparison with Duncan s method showed that the difference in expression of PIP2 and PIP1 gene in the three different times between the two dwarf and vigorous genotypes was significantly different at 1% and 5%, respectively. According to the results, the mean PIP2 and PIP1 expression in the period from April to mid-June are high, but declines over time.

Keywords:

dwarf genotypes , aquaporins , gene expression , Walnut.