Characterization and in vitro studies on the effect of SrO on nano-hydroxyapatite layer in bio-composites

Bioactive glasses (BGs) are considered as a high potential candidate in bone repair and replacement. In the present study, sol–gel derived BGs based on 60% 〖SiO〗_2-(36%-x) CaO-4%P_2 O_5-x SrO (where x=0, 5 and 10 mol%) quaternary system were synthesized and characterized. The effect of Sr substitutions on bioactivity, proliferation, alkaline phosphatase (ALP) activity of osteoblast cell line MC3T3-E1 and antibacterial activity were investigated. XRD, FTIR and SEM results confirmed the formation of nano-hydroxycarbonate apatite on the BG surfaces. The MTT and ALP activity results showed that 5 % SrO increased both differentiation and proliferation of MC3T3-E1 cells. Live/Dead and DAPI/Actin staining exhibited viable cell and the morphology of actin fibers and nuclei of MC3T3 cells. Taken together, results suggest that 58S BG with 5 mol% SrO is a good candidate for bone tissue engineering with maximum cell proliferation and ALP activity, good bioactivity and high antibacterial efficiency against MRSA.

Keywords:

Applications , Bioactivity , biocomposites , Characterization.