Evaluation of Silibinin Toxicity on Breast Cancer Cell line MCF-7

Given the prevalence of breast cancer, Mortality of patients and the public easy access to the herbal extract of milk thistle, We decided to examine the effectiveness of this plant on breast cancer cell line, MCF-7.Silibinin, a naturally occurring flavonoid antioxidant found in the milk thistle, hasrecently been shown to have potent anti-proliferative effect against various malignant cell lines. Materials and methods: In the present study, MCF-7 cells were incubated with various concentrations of silibinin for different times and survival rate and proliferation of cells after 12, 24 and 48 hours was evaluated by MTT assay. Results: The results show that Silibinin have significantly toxic effect on MCF-7 cell line. (P<0.001).So that, Silibinin decreases cell viability in a dose and time-dependent manner. Concentration 200μl of silibinin and in 48 hours had the best effect on the viability MCF-7 cells. Discussion and Conclusion: Considering to Silibinin effect on inhibiting cell growth, it could be Effective Preventer factor in the development and progression of cancer.

Keywords:

breast cancer , Silibinin , MTT assay