A novel 3D model of gastric cancer

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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ITERMED01_332

تاریخ نمایه سازی: 7 مرداد 1398

Abstract:

IntroductionClassic in vitro 2-dimensional (2D) cancer cell cultures are hypersensitive to anti-cancer drugs. So, development of three dimensional (3D) cultures to better replicate natural physiology and better predict drug efficacy, is necessary. Objectives Here we introduce a novel 3D model of gastric cancer using decellularized gastric tissue. MethodsRat gastric tissue after heart perfusion by normal saline and 1% SDS was extracted. Gastric tissue placed in SDS 1% for 3 hours and washed with PBS. MKN-45 Cells were injected into decellularized tissue specimen and cultured for 7 days. Decellularized tissue before and after cell injection examined with H&E staining and SEM. ResultsThe results showed that gastric tissue can be easily decellularized with SDS. MKN-45 cells were growth in decellularized gastric tissue easily and form tumor like structure. ConclusionDecellularized gastric tissue can be used as a proper 3D matrix for gastric cancer research

Keywords:

Three dimensional (3D) cultures , Cancer , Decellularized tissue

Authors

Akram Alizadeh

Department of Tissue Engineering, Shahrekord University of Medical Sciences, Shahrekord, Iran

Leila Rezakhani

Department of Tissue Engineering, Shahrekord University of Medical Sciences, Shahrekord, Iran

Sorayya Ghasemi

Cellular and Molecular Research Center, Basic Health Sciences, Shahrekord University of Medical Sciences, Shahrekord, Iran