Effect of pleurotus sajor-caju polysaccharide encapsulated in poly D, L lactide-co-glycolide nanoparticles for HPV vaccine in murine model
Publish place: Nanomedicine Journal، Vol: 5، Issue: 3
Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_NAMJ-5-3_004
تاریخ نمایه سازی: 18 تیر 1398
Abstract:
Objective(s): In the current work, poly D, L lactide-co-glycolide (PLGA) particles were applied for a viral vaccine for the delivery of antigens in cytosolic pathway by increasing the antigen presentation to T-lymphocytes. HPV-E7 protein with PLGA particles has been reported as a potent adjuvant for HPV vaccine by encapsulating protein into the PLGA particles. Polysaccharide from Pleurotus sajor-caju was also applied as a potent immunomodulator. Materials and Methods: HPV-E7 protein and Pleurotus sajor-caju polysaccharides (PSC) were encapsulated into PLGA nanoparticles. This combination comprised a strategy to induce helper and cytotoxic T-lymphocytes (CTL) expansion. Mice antibodies and T-lymphocyte expansion were investigated in comparison between encapsulated E7 protein into PLGA nanoparticles (E7PLGA) and E7 protein with PSC encapsulated into PLGA nanoparticles (PSC-E7PLGA). Results: The results showed that E7PLGA and PSC-E7PLGA could induce antibody response to HPV. The PSC-E7PLGA could increase the level of viral antigen-specific IgG antibodies. The cellular immune responses are also significantly enhanced by expansion of helper T-lymphocytes and CTL. PSC-E7PLGA was shown to be significantly higher in immunomodulating activity than E7PLGA. Conclusion: Thus, the encapsulation of polysaccharide and HPV-E7 antigen into PLGA nanoparticles is a strategy for development of HPV vaccine.
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Authors
Roopngam Evelyn Piyachat
Department of Clinical Immunology, Faculty of Medical Technology, Western University, Kanchanaburi, Thailand, ۷۱۱۷۰
Piyachat Evelyn Roopngam
Head of Clinical Immunology Department, Faculty of Medical Technology, Western University, T.Sralongrua, A.Huay Kra Chao, Kanchanaburi, Thailand, ۷۱۱۷۰
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