Fibroblast cells proliferation on gelatin/ olibanum electrospun nanofibrous scaffolds as an in vitro model of wound tissue engineering.

Background and Aim : In this investigation Gelatin and Olibanum powder was synthesized into fiber templates by electrospinning for wound tissue engineering applications. Electrospinning is a unique and inexpensive fabrication method which produces fibers with great similarity from the aspect of physicochemical and structural properties to the microenvironment of natural extracellular matrix. The wound healing process is influenced by fibroblast cells and extracellular matrix in the dermis and epidermis. This evaluation aimed to study the effect of the Gelatin/ Olibanum nanofibrous scaffolds on fibroblast cells behavior. Methods : Gelatin/ Olibanum nanofibrous scaffolds with different ratios were fabricated. After scaffolds sterilization with UV light for 30 minutes, the Gelatin/ Olibanum nanofibrous scaffolds were placed at the bottom of each well in a 12- well tissue culture plate and were cultured with fibroblast cells at a density of 10 3 cells/scaffold. Cells were investigated at days 1, 3, and 5 for adhesion, proliferation and viability by scanning electron microscope (SEM) and 3-[4,5-dimethylthiazol- 2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay, respectively. Results : Nanofiber diameter integrated with the high specific surface area is essential for regeneration in tissue engineering applications. The uniformity and average diameter of Gelatin/ Olibanum nanofibers decreased due to the increased in viscosity of the solutions by decreasing the content of gelatin component. SEM results denoted that bead-free Gelatin/ Olibanum nanofibers were formed. The fibroblast cells on all four types of nanofibrous scaffolds were showed to attach, spread, and proliferate well. Increment in cell growth and proliferation on the fabricated scaffolds compared to control group shows Gelatin/ Olibanum scaffold is a suitable nanofibrous substrate than 2-dimensional cell culture for cell attachment and proliferation Conclusion : The Gelatin/ Olibanum fibers indicated good biomimetic characteristics and allow promising potential for different wound healing applications. Biocompatibility and physicochemical properties of Gelatin/ Olibanum fibers would hold great potential them to become suitable candidates for wound tissue engineering.

Keywords:

Fibroblast , Nanofibers , Tissue engineering