Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

Aspergillus fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection,allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the proteinprofile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new memberof fungal annexins. This study investigates the effect of ANXC4 mutant gene in proteome profile of AspergillusFumigatus. Moreover, in order to enhance the power of protein complex separation, we used an optimizedprefractionation method. Using reverse phase-high performance liquid chromatography eight fractions wereseparated. Then to confirm the protein concentration, each fraction was tested by SDS-PAGE. Protein profile ofthese fractions was analyzed using 2-DE, and Image Master software. Among the proteins identified statistically,two emerging proteins were observed. The results show that the expression of ANXC4 could affect the expressionof some proteins in A.Fumigatus. To accurately identify these proteins, further experiments are needed includingMass spectrometry analysis.

Keywords:

Aspergillus Fumigatus , RP-HPLC , 2-DE , Prefractionation , Proteome profile