Aspartate and alanine aminotransferase activities in cultured sterlet sturgeon (Acipenser ruthenus) during overriping period

During the of reabsorbing process, oocyte cell walls are destroyed, follicular layersis dried and collapsed, and eventually destroying the tissues and cell death isobserved. This process causes a change in the amount of enzymes secreted by theorgans involved in different stages of maturation in broodstock. To do this study, 18sterlet females with an average weight of 1120.2 ± 240.4 g were selected. Bloodsampling was carried out in three groups, non-overripe, beginning overripe andcompletely overripe. IFCC method was used to determine the transferases activities.During the complete overripening, significant differences was found in case of ASTlevel so that maximum level of AST (521.33 ± 31.16 IU/L) was seen in the completeoverripening stage and the lowest level (389.16 ± 30.8 IU/L) was observed in nonoverripeningfish. However, the activity of ALT enzyme showed no significantdifference among the fish (P> 0.05). This study showed overripening can playsignificant role in liver enzymes fluctuation of oocytes. Nevertheless, the use of thesefactors as biomarkers to control the timing of reproduction in cultured sterlet females’broodstock could be possible.

Keywords:

Oocyte , Over ripening , Sterlet sturgeon , Liver enzymes