Comparison of Different Extracts of Nettle in Quercetin and Evaluation of its Antimicrobial Activity
Publish place: Chemical Methodologies، Vol: 4، Issue: 5
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_CHM-4-5_004
تاریخ نمایه سازی: 1 مرداد 1399
Abstract:
Nettle (Urtica dioica L.) is one of the most well-known medicinal plants. It has been mostly used for medical purposes. This study summarizes extensive results on the antimicrobial effect of Urtica dioica L. The aim of this study was to investigate several extraction methods of nettle extract for more access to flavonoid materials especially quercetin. For this purpose, three methods (soaking, sonication, soxhlet) and three solvents (chloroform, methanol, deionized water) were used for extraction. Finally, the highest amount of quercetin determined by HPLC was sonication for 90 min with methanol solvent. The effect of this extract was investigated on several pathogenic microbes including Escherichia coli, Entrecoccus faecalis, Klebsiella pneumoniae, Acinetobacter calcoaceticus, Candida albicans. Antibiogram testing revealed that Escherichia coli and Candida albicans were resistant to nettle extract, Acinetobacter calcoaceticus and Klebsiella pneumonia, were sensitive and Enterococcus faecalis was semi-susceptible.
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Authors
Mahsa Taghvaei
Department of Biochemistry, Payame Noor University, ۸۱۵۸۱-۸۴۴۳۱, Isfahan, Iran
MohamMad Fazilati
Department of Biochemistry, Payame Noor University, ۸۱۵۸۱-۸۴۴۳۱, Isfahan, Iran
Habibollah Nazem
Department of Biochemistry, Payame Noor University, ۸۱۵۸۱-۸۴۴۳۱, Isfahan, Iran
Saeed Habibollahi
Department of chemistry, Payame Noor University,
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