کاربرد تکنیک ‭STG)Shoot- tip grafting)‬در تولید نهالهای سالم و عاری از بیماری ویروسی تریستزا در پرتقال رقم واشنگتن ناول

For the first time, shoot- tip grafting was carried out by Murashigue et a1. in 1972. Then Navarro et aLl97S. developed this technique and it was used instead of nucellIar, thennotherapy, apical bud culture and etc.Now, This method is used to produce virus and virus-like free plants.This study was carried out for produce CTV free plants. Shoot-tip of infected Washington nave' orange by tristeza containing meristem and two leaf primordia was excised (0.2 - 0.4 mm). It was grafted on Troyer Citrange and Citrumelo seedlings by two methods, inverted T -budding and in contact with the vascular ring. These seedlings had 3-5 cm length. grafted seedlings were planted on liquid medium and incubated on growth chamber in 27 c and 16 hours photoperiod with 1000 lux illumination. if grafting was successful, plants were transplanted dn suitable soil. Shoot-tip grafted plants were tested by DAS ELISA against tristeza virus. All of them rere not infected. In this research, we used factorial design based on complete randomized design with four treatments and four replications. Each treatment had 10 plantlets. The results showed that inverted T- budding was more successful. There was no significant differences between used root stocks.

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پرتقال واشنگتن ناول , تریستزا , ریزپیوندی نوک شاخه , مرکبات , گیاهچه ترویر سیترنج , گیاهچه سیتروملو