Investigation the elicitation of inflammatory responses by Resilon and guttaperchaon human monocyte cell line THP-1
Publish Year: 1399
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICRSIE05_034
تاریخ نمایه سازی: 1 آبان 1399
Abstract:
Objective: In this in vitro study, the induction of inflammatory responses to the filler materials which usedtoday (Resilon and Gutta-percha) was investigated on the human monocyte cell line THP-1.Methods: Three millimeters of gutta-percha and Resilon were placed in direct contact with the THP-1 cellsuspension after disinfection in sodium hypochlorite solution on the ninety-six plates. Root filler materialswere evaluated for both LPS and non-LPS. The positive control group contained LPS, and the negativecontrol group contained only the culture medium containing the cell. Production of IL-1β and TNF-α afterexposure to the tested materials was evaluated by ELISA.Results: Production of IL-1β in all groups increased as time-dependent. Gutta-percha's ability to produceIL-1β was greater than Resilon. In both gutta-percha and Resilon groups, LPS contamination increasedTNF-α production.Conclusion: There was no statistically significant difference in Resilon and gutta-percha elicitsinflammatory cytokine production (either in terms of and gene expression or protein synthesis).
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Authors
Nasrin Hadi
Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan,Iran
Marjan Sadat Hosseini
Department of Genetics, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Faezeh Namazi
Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran