Detection of aacc1 and aacc2 Genes in Clinical Isolates of Klebsiella Pneumoniae

Introduction: Klebsiella pneumoniae is one of the main agent of nosocomial infections. Reports around the world emphasize on the resistance to aminoglycoside antibiotics in recent years. The purpose of this study is to determine the frequency of aacC1 and aacC2 genes in clinical isolates of Klebsiella pneumoniae.   Methods: A total of 100 Klebsiella   pneumoniae were collected from tertiary university hospitals, Khorramabad city, Iran, from February to August 2018. The obtained samples were identified by standard biochemical and microbiological tests. Susceptibility pattern of isolates were determined according to Clinical Laboratory Standards Institute (CLSI) advices using disk diffusion method. After DNA extraction, all Klebsiella   pneumoniae isolates were evaluated for the presence of aacC1 and aacC2 genes using PCR assay.   Findings: Out Of 100 Klebsiella pneumonia isolates the highest resistance was related to kanamycin (35%), tobramycin (29%), and amikacin (23%). The aacC1 and aacC2 genes was detected in 22.8 and17.2 percent, respectively.   Conclusion: Our results indicate that the prevalence of the aacC1 and aacC2 genes was high and it is clear that we witness an increase in resistance to antibiotics in clinical isolates. Therefore, we expect an increase in the resistance to aminoglycoside antibiotics in the near future.

Keywords:

Klebsiella pneumoniae , PCR assay , aacC1 , aacC2.