Resveratrol increases the sensitivity of breast cancer MDA-MB-231 cell line to cisplatin by regulating intrinsic apoptosis
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 24، Issue: 1
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJBMS-24-1_010
تاریخ نمایه سازی: 19 دی 1399
Abstract:
Objective(s): Breast cancer is one of the most common types of cancer. Chemotherapeutic agents used during treatment induce cytotoxic effects also on normal cells in the tissues. Anti-oxidants used in combination with chemotherapeutic agents have been shown to reduce toxicity on normal cells to a minimum, and some anti-oxidant substances have chemotherapeutic effects. Cisplatin (CDDP) is a platinum class drug that is used clinically in the treatment of many cancers. Resveratrol (RSV) is a natural polyphenol with potent anti-oxidant and anticancer properties. In this study, we aimed to investigate apoptotic effects of using cisplatin and RSV alone or in combined treatment of MDA-MB-231 cells.Materials and Methods: The cytotoxic effects of the drugs on MDA-MB-231 cells were determined by MTT method. Subsequently, the change in CDDP-induced apoptotic effect after RSV addition was examined using the AnnexinV FITC labeling, and TUNEL staining method. Activation of caspase-9, -3 in MDA-MB-231 cells was measured by flow cytometer. The mitochondrial membrane potential (MMP), the major factor on the intrinsic pathway, was measured using flowcytometry. Results: The combined dose (23 μM CDDP + 72 μM RSV) produced more cytotoxicity than the agents used alone, leading to early apoptosis (8.2%), 31% depolarization, and 23% DNA fragmentation. Caspase-9 was found to be 30.5% in this combined group and caspase-3 was 26.3%. Conclusion: RSV, an effective anti-oxidant, and CDDP as an effective drug in cancer treatment, were found to increase apoptosis when given in the MDA-MB-231 cell.
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Authors
Filiz Ozdemir
Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
Arda Sever
Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
Yüksel Öğünç Keçeci
Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
Zerrin Incesu
Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
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