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Title

Comparative Study of Garlic Extract Effect on the Expression of Genes Involved in Quorum Sensing in Pseudomonas aeruginosa and the Antibiotic Tobramycin

Year: 1399
COI: JR_IJMM-15-1_006
Language: EnglishView: 106
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Authors

Mansoureh Ghods Zadeh - Master of Microbiology, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
Soheila Moradi Bidhendi - Associate Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
Fatemeh Ashrafi - Assistant Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract:

Background: The expression of most genes involved in pathogenesis in Pseudomonas aeruginosa is controlled and regulated by a gene system called quorum sensing (QS). The purpose of this study was to investigate the quorum quenching of garlic extract in preventing the expression of genes involved in QS system of P. aeruginosa. Methods: In the present cross-sectional study, 12 P. aeruginosa strains were collected from burn wounds, cultured on special media and then confirmed by differential tests. The PCR test was performed to detect the lasI and lasR genes. The expression level of lasI gene was assessed in the presence of garlic extract and tobramycin antibiotic by real-time PCR, and the active ingredient of garlic extract was analyzed by HPLC. Results: Examination of 12 strains cultured on special and differential media showed that all 12 strains were P. aeruginosa. The PCR results indicated that all strains had 100% lasI and lasR genes. The real-time PCR results also revealed that the garlic extract was able to reduce the expression level of lasI gene in P. aeruginosa. It was found that tobramycin has a greater ability to reduce the expression level of this gene compared with garlic extract. Conclusion: This study showed that the garlic extract could reduce the expression of lasI gene in P. aeruginosa isolates, and that the active ingredients of this extract could be used to treat infections as an alternative to antibiotic therapy.

Keywords:

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This Paper COI Code is JR_IJMM-15-1_006. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

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Ghods Zadeh, Mansoureh and Moradi Bidhendi, Soheila and Ashrafi, Fatemeh,1399,Comparative Study of Garlic Extract Effect on the Expression of Genes Involved in Quorum Sensing in Pseudomonas aeruginosa and the Antibiotic Tobramycin,https://civilica.com/doc/1159266

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  • Waters CM, Bassler BL. Quorum sensing: cell-to-cell communication in bacteria. ...
  • Papenfort K, Bassler BL. Quorum sensing signal-response systems in Gram-negative ...
  • Solano C, Echeverz M, Lasa I. Biofilm dispersion and quorum ...
  • Trappetti C, Oggioni MR. Biofilm Formation Under In Vitro Conditions. ...
  • Oliver A, Mulet X, López-Causapé C, Juan C. The increasing ...
  • Brackman G, Coenye T. Quorum sensing inhibitors as anti-biofilm agents. ...
  • Truchado P, Larrosa M, Castro-Ibáñez I, Allende A. Plant food ...
  • Bergey DH, Hendricks D, Holt JG, Sneath PH. Bergey's Manual ...
  • Bratu S, Gupta J, Quale J. Expression of the las ...
  • O'Loughlin CT, Miller LC, Siryaporn A, Drescher K, Semmelhack MF, ...
  • Zhou Y, Kong Y, Kundu S, Cirillo JD, Liang H. ...
  • Narusaka M, Shiraishi T, Iwabuchi M, Narusaka Y. rpoD gene ...
  • Lewis K. Platforms for antibiotic discovery. Nature reviews Drug discovery. ...
  • Reiter J, Hübbers AM, Albrecht F, Leichert LIO, Slusarenko AJ. ...
  • Dwivedi VP, Bhattacharya D, Singh M, Bhaskar A, Kumar S, ...
  • Choo S, Chin VK, Wong EH, Madhavan P, Tay ST, ...
  • Schuster M, Lostroh CP, Ogi T, Greenberg EP. Identification, timing, ...
  • Smith RS, Harris SG, Phipps R, Iglewski B. The Pseudomonas ...
  • Bazargani MM, Rohloff J. Antibiofilm activity of essential oils and ...
  • Bjarnsholt T, Jensen PØ, Rasmussen TB, Christophersen L, Calum H, ...
  • Fulghesu L, Giallorenzo C, Savoia D. Evaluation of different compounds ...
  • Rasmussen TB, Bjarnsholt T, Skindersoe ME, Hentzer M, Kristoffersen P, ...
  • Bodini S, Manfredini S, Epp M, Valentini S, Santori F. ...
  • Harjai K, Kumar R, Singh S. Garlic blocks quorum sensing ...
  • Annapoorani A, Umamageswaran V, Parameswari R, Pandian SK, Ravi AV. ...

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Scientometrics

The specifications of the publisher center of this Paper are as follows:
Type of center: Azad University
Paper count: 8,299
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