Effect of water temperature on hemocytes and histopathological findings in freshwater Crayfish (Astacus leptodactylus)

In  this  research,  the  effect  of  different  water  temperature  on  THC  (Total  hemocyte  count),  variety  of  hemocyte  cells  and  histopathological  differences    in  Crayfish  Astacus leptodactylus  were  studied.  This  study  was  designed  in  three  groups  in  triplicate  with  20  Crayfish  in  each  glass  aquarium.  The  rate  of  temperature  in  three  groups  were  10,15,  20,  and  25 °C, respectively.  The  results  indicated  that  the  differences  of  THC  value  between  Group1  (69±  19.00)    and  Group  3  (18±  12.49)  were  significant  after  48  h  .  After  168  h,  also  the  differences  of  THC  value  between  Group  3  (9±  6.24)  with  Groups 1 & 2  (35.33±  7.02 &32.33±  8.73)  were  significant.  The  highest  and  lowest  density  of    hemocyte  cells  were belong to semigranular  cells  (SGC),  granular  cells  (GC)  and  hyaline  cells  (HC), with estimated values of  59-64%,  28-36%  and  3-8%  respectively.  The  result  of  histopathology  in  heaptopancreas,  gill  and  heart  in  Group 3  affecting  of  high  temperature  showed  the  distribution  of  hemocyte  aggregation  and  pyknosis  of  nucleus  within  vaculation  of  hepatopancreas.  In  Groups  1  and  2  the  differences  of  hepatopancreas  and  gill  lamella  were  lower  and  no  pathological  changes  of  heart  were  observed. No significant changes of digestive tract were observed in all treatments.

Effect of water temperature on hemocytes and histopathological findings in freshwater Crayfish (Astacus leptodactylus) Keywords:

Astacus leptodactylus , Total hemocyte count , Histopathology