Di-ethanolamine Might Cause Bone-related Complications Due to the Reduction of Osteogenic Differentiation and Induction of Oxidative Stress
Publish place: Journal of Genetic Resources، Vol: 6، Issue: 2
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
View: 181
This Paper With 10 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_SGR-6-2_009
تاریخ نمایه سازی: 6 اردیبهشت 1400
Abstract:
Di-ethanolamine (DEA) is a well-known environmental pollutant used in manufacturing soap, detergent, body lotion, and other sanitary products. DEA has been reported to cause cytotoxicity in different tissue and cell, but no study was found to explain the toxic effect of DEA on rat bone marrow mesenchymal stem cells (BMSCs) differentiation. Thus in the present study, the differentiation property of BMSCs treated with DEA was investigated. BMSCs after ۳rd passage were cultured in osteogenic media in presence of ۱ and ۴ mM of DEA for ۲۱ days. Then, the viability, based on ۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵-diphenyltetrazolium bromide or MTT) and morphology of nuclei and cytoplasm (using fluorescent dye), as well as osteoblasts mineralization property (based on quantitative alizarin red and calcium concentration), were studied. Also, sodium and potassium level, the activity of alanine transaminase, aspartate transaminase, alkaline phosphatase, and lactate dehydrogenase (LDH) were determined. The level of total-antioxidant capacity (TAC), malondialdehyde (MDA), and the activity of antioxidant enzymes (superoxide dismutase and catalase) also were estimated. The DEA treated cells showed nuclear enlargement and cytoplasm shrinkage as well as an increase in potassium level. Also based on LDH activity elevation, we observed a cellular anaerobic metabolism. Also, a significant increase in MDA was shown, while TAC and antioxidant enzyme activity was reduced. Finally, a significant decreased in cell viability and differentiation ability of BMSCs was observed. Since BMSCs are the cellular backup to generate osteoblasts, therefore its intoxication with DEA might cause bone complications, thus we recommend, prevention of DEA utilization in health-related products.
Keywords:
Authors
Mohammad Hussein Abnosi
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran
Setarehsadat Hosseini
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :