Probing the effects of synthesized silver nanowire/reduced graphene oxide composites on the structure and esterase-like activity of human serum albumin and its impacts on human endometrial stem cells: A new platform in nanomedicine

Objective(s): Nowadays, the unique and fascinating properties of graphene‐based nanocomposites make them one of the most promising materials for therapeutics, delivery carriers as well as tissue engineering. On the other hand, silver nanowire has been attracting more attention in nanomedicine applications, too. In this study, the effects of synthesized silver nanowire/reduced graphene oxide (AgNWs/rGO) composites on the structure and esterase-like activity of Human Serum Albumin (HSA), as well as its impacts on Human Endometrial Stem Cells (hEnSCs), were evaluatedMaterials and Methods: AgNWs/rGO composite was first synthesized and fabricated. Subsequently, its effects on the structure and esterase-like activity of HSA were evaluated by UV-Visible spectroscopy, circular dichroism spectroscopy, and fluorescence spectroscopy. Afterward, its impacts on the viability and growth of hEnSCs were studied by MTT assay, DAPI staining, and flow cytometry analysis.Results: The spectroscopic results showed that AgNWs/rGO composite could form a complex with HSA, however, did not affect the secondary structure of HSA and the binding constant for this complex was found to be ۵.۴×۱۰۴ mL.mg-۱. Furthermore, HSA maintained most of its activity in the presence of the AgNWs/rGO composite. Based on FRET (fluorescence resonance energy transfer) data the value of r۰ was less than ۷ nm signifying that the energy transfer from HSA to AgNWs/rGO composite occurs with a high level of possibility. The MTT assay, DAPI staining, and flow cytometry analysis indicated that the AgNWs/rGO composite was non-toxic towards hEnSCs. Conclusion: Our results suggest that the prepared AgNWs/rGO composite, potentially, is suitable in nanomedicine applications such as tissue engineering and drug delivery.

Keywords:

Esterase-Like Activity , Human Endometrial Stem Cells , Human Serum Albumin (HSA) , Silver nanowire/reduced graphene oxide , Zeta Potential