The Effect of Concurrent Injection of Amantadine and Paroxetine on Positive Reinforcing Effect of Morphine in Conditioned Place Preference(CPP) Model in Mice.
Publish Year: 1386
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ZUMS-15-59_004
تاریخ نمایه سازی: 11 اردیبهشت 1400
Abstract:
Background and Objective: Increased level of dopamine in accumbens nucleus has a key role in the rewarding effects or positive reinforcement of abused drugs, whereas serotonin facilitates dopamine release in brain .The aim of this study was to investigate the effect of concurrent use of amantadine and paroxetine on reinforcing effect of morphine in conditioned place preference model in mice.
Materials and Methods: In this experimental study male NMRI mice (۲۰-۳۰ g) were used within ۶ consecutive days including preconditioning ,conditioning and postconditioning phases. On the first day, after removal of the partitions, time spent in every ۳ compartments was measured for ۱۰ minutes. After determination of low and high preferred side, animals received morphine sulfate (۵ mg/kg ) intraperitoneally on the ۲nd and ۴th days in the least preferred side, but on the ۳rd and ۵th days of the test, animals received saline (۱۰ml/kg) in high preferred side. On the test day or postconditioning phase, animals received amantadine, and paroxetine alone or their concurrent does, instead of morphine. Control group received saline in both sides (n = ۸).
Results: Our results show that morphine significantly and dose dependently ( ۲.۵, ۵,۱۰ mg/kg) induced CPP (P<۰.۰۰۱). Amantadine, only in doses of ۵ and ۱۰ mg /kg (P<۰.۰۱, P<۰.۰۰۱, respectively) induced CPP. Paroxetine induced CPP in all doses. Concurrent use of amantadine(۱۰mg/kg) and paroxetine (۱۰mg/kg) significantly enhances morphine - like CPP ( P <۰.۰۰۱).
Conclusion: Concurrent use of drugs, releases dopamine and inhibit reuptake of serotonin, and may potentiate morphine-like CPP and could be useful in decreasing some opioid withdrawal signs.
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Authors
سید عباس ملکی
Department of Pharmacology, School of Veterinary Medicine, Tehran Azad University, Sciences and Research Branch, Tehran, Iran