The Efffect of Titanium on Stearic Acid Transport in Rat Everted Gut Sac

Background and Objective: An everted intestinal sac (EGS) technique has been used to extensively estimate the transport and intestinal absorption in rats. Therefore, a number of factors such as pH and the nature of solvent may play an important role in fatty acid uptake by entrocytes. There are reports indicating that fatty acid transport is affected by many biochemical parameters including trace elements. In this study the effect of titanium on stearic acid transport was investigated. Materials and Methods: Wistar male rats (۲۰۰-۲۵۰gr) were used for the experiments. Rats were killed, their intestine were removed and the jejunum parts were dissected. Everted gut sac was prepared from these parts. Sacs full of buffer were incubated in a medium containing stearic acid and titanium. Then the transported stearic acid inside the EGS was measured by spectrophotometer under different conditions of temperature and concentrations. Data was analyzed using SPSS software and Mann Whitney test. Results: The results showed that titanium decreased fatty acid uptake by entrocytes in a dose dependent manner. Titanium concentrations of ۰.۵, ۱, ۱.۵ and ۱۰ micromoles in the presence of sodium chloride can decrease the uptake of stearic acid by ۳۲.۲%, ۴۳.۴%, ۵۴.۵% and ۶۱.۵%, respectively. Titanium concentrations of ۰.۵, ۱, ۱.۵ and ۱۰ micromoles in the absence of sodium chloride in medium could decrease the stearic acid by ۱۰%, ۱۹.۵%, ۲۳.۹% and ۲۸.۳% respectively which is not very affective. Conclusion: Stearic acid transport appeared to be a Na dependent process and titanium may exert its inhibitory effect by interfering with this system. Our results showed that the incubation time, stearic acid concentration and pH were effective on stearic acid transport. Titanium decreased stearic acid transport in Rat EGS. This should be considered seriously, especially in people exposed to titanium compounds for a long period.

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Keywords: Stearic acid , Titanium , Entrocyte