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Title

Comparison of the Genetic Convergence Degree of Mycobacterium Tuberculosis (TB) Strains Isolated from Patients Infected with TB by MIRU-VNTR Technique

Year: 1390
COI: JR_ZUMS-19-75_004
Language: EnglishView: 114
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Authors

مهدی جعفریان - Islamic Azad University, Jahrom Branch, The Research Center of Mycobacteriology, Jahrom, Iran
پریسا فرنیا - Mycobacteriology Research Center , Shahid Beheshti University of Medical Sciences, Tehran, Iran
محدثه مظفری - Qom Azad University of Medical Sciences, Qom, Iran
مجتبی احمدی - Mycobacteriology Research Center , Shahid Beheshti University of Medical Sciences, Tehran, Iran
محمدرضا مسجدی - The Research Center of TB and Lung Diseases, Shahid Beheshti University of Medical Sciences. Tehran, Iran
علیاکبر ولایتی - The Research Center of TB and Lung Diseases, Shahid Beheshti University of Medical Sciences. Tehran, Iran

Abstract:

Background and Objective: In recent decades, epidemiology has significantly been considered in hygienic studies and disease control, and has made a way into all the programs and hygiene policies. By examining the convergence of harmful lineage genetic patterns, the common infectious resources among the patients can be inferred. The purpose of this study was to compare the Mycobacterium Tuberculosis genetic patterns convergence isolated from patients infected with Mycobacterium Tuberculosis by MIRU-VNTR technique. Materials and Methods: After isolation the samples from Lowenstein Jensen culture environment and taking segregate tests and drug susceptibility, the DNA was extracted using CTAB/Nacl technique. The genetic patterns of lineages were calculated according to ۱۲ loci format with MIRU-VNTR technique. Demographic and molecular information of patients was used for epidemiological purposes. Results: After performing drug sensitivity test, ۶۵/۱۴۰ (۶۴/۴%) samples fall into MDR, ۲۹ (۲۰/۷%) samples in non MDR category, and the rest of them were among drug – sensitive lineages. Lineage genetic pattern analysis indicated that ۴۹ (۳۵%) of samples related to Delhi/CAS, ۲۸ (۲۰%) to Uganda I, ۱۶ (۱۱/۴%) to New I, ۱ (۰.۷%) to EAI, ۳(۲/۱%) to Haarlem, and ۵(۳/۵%) to H۳۷RV families. Conclusion: The genetic pattern convergence comparison exhibited that the most common and variant genetic patterns was seen in Tehran province which were mostly connected to south (from the South of Tehran to Azadi Square) and to the border cities neighboring Afghanistan, Iraq, Turkmenistan and cities with extreme percentage of immigration, all of which signified shared polluted resources among patients.

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This Paper COI Code is JR_ZUMS-19-75_004. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

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جعفریان, مهدی and فرنیا, پریسا and مظفری, محدثه and احمدی, مجتبی and مسجدی, محمدرضا and ولایتی, علیاکبر,1390,Comparison of the Genetic Convergence Degree of Mycobacterium Tuberculosis (TB) Strains Isolated from Patients Infected with TB by MIRU-VNTR Technique,https://civilica.com/doc/1191313

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Scientometrics

The specifications of the publisher center of this Paper are as follows:
Type of center: Azad University
Paper count: 1,150
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