The influence of Topical Treatment of Dermal Wounds with Silver Nanoparticles on ALT and AST Enzymes and Hemoglobin in Mice (Mus Musculus)

Background and Objective: Silver nanoparticles (Ag-NPs) have long been used to treat acute and chronic wounds. Very little attention has been paid to the toxicity of this nanoparticle. In this study, the possible toxicological effects of nanosilver particles, was assessed by determining the enzyme levels of liver along with hemoglobin over the period of skin wound healing in mice. Materials and Methods: A group of ۵۰ Balb/c mice of about ۸ weeks (weighting ۲۴.۲±۳.۰ g) were randomly divided into two groups: Ag-NPs and control groups, each with ۲۵ mice. After creating the equal wound on the back of all animals, the wound bed was treated in the Ag-NPs group with a volume of ۵۰ microliters from the nanosilver solution (۱۰ppm), and in the control group with the same amount of distilled water. The experiment lasted for ۲۱ days. On days ۲, ۷, ۱۴ and ۲۱ of the experiment the serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and hemoglobin were measured. Statistical analysis was performed using independent t-test. Results: Results showed that AST, ALT and hemoglobin levels were not significantly different between Ag-NPs and the control groups. Conclusion: The study suggests that treatment of wounds with silver nanoparticles may not produce toxicological effects on hemoglobin and liver function in mice (Mus musculus) as indicated by measurment of serum levels of AST and ALT enzymes.

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Keywords: Wound healing , Silver nanoparticles , ALT , AST , Hemoglobin