Frequency of Class I and II Integrons in Metalobetalactamase Producing Clinical Isolates of Pseudomonas Aeruginosa
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نوع سند: مقاله ژورنالی
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JR_ZUMS-22-94_011
تاریخ نمایه سازی: 11 اردیبهشت 1400
Abstract:
Background and Objective: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen. Evidence suggests that the incidence of enzyme-producing strains of Pseudomonas aeruginosa Metalo Beta Lactamases (MBL) is a major problem in the treatment of infections caused by this organism. The aim of this study was to investigate the frequency of class I and II integrons among metalobetalactamase producing Pseudomonas aeruginosa isolated from clinical samples from Zanjan hospitals. Materials and Methods: In this cross-sectional study, ۱۲۰ Pseudomonas aeruginosa isolates from clinical specimens were collected from Zanjan hospitals in ۲۰۱۲-۲۰۱۳. After verifying isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Baur method) was performed according to CLSI guidelines against ۷ antibiotics. The DDST was then carried out for detection of MBLs. Finally, the presence of IntI۱, IntI۲, blaVIM and blaGIM genes was determined by PCR. Results: In our study, the highest and the lowest resistant patterns were related to Cefotaxime and Amikacin with ۴۳.۳% and ۲۱.۶%, respectively. Of ۱۲۰ isolates, the metallo- beta-lactamase was detected in ۳۵ (۲۹%) isolates. According to results, the frequency of intI۱, intI۲, blaVIM genes in MBL producers were ۳۲(۹۱%), ۳(۹%), and ۶(۱۷%), respectively, but no blaGIM positive isolate was detected. Also all of IntI۲ positive isolates carried IntI۱ and blaVIM,, simultaneously. Conclusion: The results of this study indicate high frequency of class I integron gene among MBL producing Pseudomonas aeruginosa isolates. Due to the importance of integrons in dissemination of antibiotic resistance genes, detection and prevention of these elements are necessary. References ۱- Kohlenberg A, Weitzel-Kage D, van der Linden P, et al. Outbreak of carbapenem-resistant Pseudomonas aeruginosa infection in a surgical intensive care unit. 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Authors
ناهید کرامتی
Dept. of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran
حبیب ضیغمی
Dept. of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran
فخری حقی
Dept. of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran