The Effect of Hydroalcoholic Extracts of Dorema aucheri, Stachys pilifera and Trachyspermum copticum on Proliferation of Rat Brain Oligodendrocytes

Background and Objective: Dorema aucheri, Stachys pilifera and Trachyspermum copticum are used in Iranian traditional medicine especially in the province of Kohgilouyeh and Boyerahmad. This study aimed to determine the effect of these plants on proliferation of rat brain oligodendrocytes. Materials and Methods: Macerated hydroalcoholic extract of each plant was prepared using ۵۰% ethanol. The OLN-۹۳ oligodendrocytes were incubated for ۲۴ h with different concentrations of these extracts. Then, percentage of viable cells was determined using a method based on the reduction of tetrazolium salt. Results: Extract of Dorema aucheri at ۵۰, ۱۰۰, ۲۰۰, and ۴۰۰ µg/ml decreased viable cells from ۱۰۰ ± ۴% (control cells) to ۸۴ ± ۴, ۵۸ ± ۶ (P<۰.۰۰۱), ۶ ± ۰.۵ (P<۰.۰۰۱), ۴ ± ۰.۵% (P<۰.۰۰۱), respectively. Similarly, Stachys pilifera extract at ۵۰, ۱۰۰, ۲۰۰ and ۴۰۰ µg/ml decreased viable cells from ۱۰۰ ± ۴% to ۸۱ ± ۶, ۶۴ ± ۳ (P<۰.۰۱), ۶۱ ± ۴ (P<۰.۰۱), ۵۵ ± ۲% (P<۰.۰۰۱), respectively. Also, Trachyspermum copticum extract at concentrations higher than ۵۰ µg/ml significantly decreased percentage of viable cells. Conclusion: Extracts of Dorema aucheri, Trachyspermum copticum and Stachys pilifera reduce proliferation of rat brain oligodendrocytes.

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Keywords: Dorema aucheri boiss , Stachys pilifera , Trachyspermum copticum , Oln-۹۳ cell line , Antiproliferative effect