To study anticancer effect of Syzygium cumini in HT-۲۹ colorectal cancer cell line in approach to Bax and bcl۲ genes

Background and Aim: According to WHO (World Health Organization) cancer is one of the leading cause of death worldwide. Out of all type of cancers colorectal cancer is ranked ۳rd after lung and breast cancers and ahead of prostate, non-melanoma skin cancer and gastric cancers. Still conventional therapies like surgery, chemo and radiotherapy are widely used options. Hence, finding safer compounds with least side effects to combat can.cer is main aim of researchers in field of drug design, molecular genetics and biomedicine. Plant derived compounds are ideal options as they might have better outcome with minimal side effects.Methods: In the current research, the anticancer effect of Syzygium cumini plant ethanolic extract (SCE) was assessed on HT-۲۹ CRC model cell line. In this regard, effects of SCE were evaluated on the apoptosis and proliferation of HT-۲۹ cells by (MTT) assay, flow cytometry and Annexin V/PI. Behind that, expression ration of genes involved in process of apoptosis has been studied, including: Bax, Bcl-۲, Caspases such as ۳,۸ and ۹, matrix metalloproteinase -۱۳, and microRNAs like miR-۲۱ and miR-۳۴a were measured by qRT-PCR.Results: According to the MTT test, SCE suppress the proliferation of HT-۲۹ cells drastically. The flow cytometry analysis also elucidated a significant rate of induced apoptosis after SCE treatment. Expression analysis of the desired genes also changed after treated by SCE.Conclusion: The outcome of this study suggests that syzygium cumini might be contemplate as a future chemotherapeutic agent and suitable candidate for in vivo trial.

Keywords:

Apoptosis , bax , bcl۲ , Colorectal cancer , chemotherapeutic agent , HT-۲۹ , Syzygium cumini