Evaluation of alternations in AkT gene expression in the T۹۸G Glioblastoma cell line under treatment with thiosemicarbazones complexes (Ni)

Background and Aim: Primary brain tumors originate from neuroepithelial tissue (astrocyte-oligodendrocyte-microglia-ependymal cells, etc.) and usually appear in the posterior cranial cavity in children and in the anterior two-thirds of the adult cerebral hemisphere. However, these types of brain tumors can affect any part of the brain as well. Glioblastoma multiforme is the most common and malignant primary brain tumor. One of the promising sites in which thiosemicarbazones compounds are being developed is their use against cancer. Their antitumor activity is very distinct and highly dependent on the biological type of tumor cells.The purpose of this study was to Evaluation of alternations in AkT gene expression in the T۹۸G Glioblastoma cell line under treatment with thiosemicarbazones complexes(Ni)Methods: In this study, Ni thiosemicarbazones complexes were prepared with ۱۰۴ and ۱۰۰.۵µM concentrations. The adenocarcinoma T۹۸G cell line was treated by Ni in these groups after cell passage. Then RNA extraction and cDNA synthesis were performed and the expression of Akt gene in the PI۳ Kinase / AkT signaling pathway and GAPDH gene as the housekeeping gene were evaluated by Real Time PCR. Statistical analysis was performed.Results: Our results confirmed that, at both concentrations of ۱۰۰.۵ and ۱۰۴ µM a considerable gene expression decreasing observed after ۲۴ hours treatment. At ۱۰۰.۵ and ۱۰۴ µM concentrations, expression was detected ۴.۴۵۲ and ۳.۹ fold respectively.Conclusion: The results of trials related to thiosemicarbazones complexes Ni and comparison with control drug showed that Ni can be effective in reducing Akt gene expression as an oncogene.

Keywords:

Glioblastoma multiforme , thiosemicarbazones complexes , T۹۸G , AKT