Study of the vitality of frozen blastema tissue

Background and Aim: Nowadays, researchers are looking for study models for laboratory research, including Animal Modeling, Tissue Modeling, and Cell Modeling. According to the results of the studies, the blastema tissue contains a group of undifferentiated cells that create in parts of the body of alive creature. The purpose of this study is to find a suitable model for the maintenance of tissue bank as well as a proper model (tissue modeling) for specialized laboratories.Methods: In this investigation, the four New Zealand ۶-month-old male rabbits were used according to standard conditions. Seven ۲-mm punches were created per rabbit's pinna at the distance of the marginal and central veins. Two days later, a ۴ mm diameter punch was performed again. After adding blastema rings to the cryotubes containing the culture medium, the cryotubes were placed in the freezer at -۸۰ ° C for one week and then transferred to a liquid nitrogen tank. Four blastema rings were also put in complete culture medium as control samples. Blastema rings were investigated in two experimental groups. The first and the second group was kept in liquid nitrogen for ۱۵ days and one month, respectively. After the defrost technique, the samples were cultured. On days ۷, ۱۰, ۱۵, and ۲۱ after culture, the vitality of the blastema cells was assessed by trypan blue color and with invert microscope.Results: According to the investigations, there were significant differences between the test and control samples based on the evaluation index, which is the cell vitality. Also, after the ۱۰th day of culture, in the tissue of control blastema, the cells collapse was observed at the culture dish surface. Accordingly, this research is performed using other cryopreservation protocols, which, comparatively, the relevant results will be reported in the original article.Conclusion: According to the results, it seems that ۱۵ days of freezing time and lower concentrations of DMSO, increase the probability of the vitality of blastemal resulting from auricle punch of the rabbit as a tissue model.

Keywords:

Tissue Freezing , Tissue Culture , Cell Vitality