Effect of Ergolide from inula oculus-christi on apoptosis and cell cycle mechanism in Breast cancer cell line

Background and Aim: Ergolide belongs to the family of Asteraceae and it was traditionally wide used in treatment of kidney stones and urethra infection; besides, recently the potent sesquiterpene Ergolide isolated from inula species has gained increasing attention in cancer treatments.Methods: Viability, Metabolic Activity, Proliferation evaluated using Trypan blue, MTT assay, Flow cytometery and Acridine Orange respectively. Moreover, qRT-PCR was performed to examine the expression of P۲۱, P۲۷, BCL-۲, Sirt۱, Caspase۳ and Atg۱۰.Results: Ergolide exert cytotoxicity against MCF-۷ and MDA-MB-۴۶۸ human breast cancer cells. It also caused ROS production (increased Sirt۱ expression) during early hours after treatment of cells which was then followed by cell cycle arrest in G۰/G۱ (increased expression of P۲۱ and P۲۷) phase and autophagy induction (expression Atg۱۰ increased). Also, the expression of BCL-۲ and Caspase۳ reduction and increased respectively.Conclusion: This study demonstrated that Ergolide was capable to extend chemotherapeutic sensitivity in MCF-۷ and MDA-MB-۴۶۸ human breast cancer cells by reducing BCL-۲ expression. Therefore, it might act as a promising anticancer agent for the treatment of Breast cancer.

Keywords:

Ergolide , MCF-۷ , MDA-MB-۴۶۸ , Apoptosis